COVID-19 & Genetics: Who gets sick and why?

Genetic variants play a role in susceptibility to infectious diseases. Not everyone will get the norovirus or a particular strain of the flu — due to genetic variants. New research shows that genetics also plays a role in the severity of COVID-19.

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Genetics and COVID-19: Who gets sick?

Before we begin: Research on COVID-19 is being produced at a record pace, which is great. But, not all publications are relevant or good sources of information. As software developers like to say: ‘garbage in, garbage out‘. With poor data and flawed models, many of the research studies being churned out on COVID-19 fall in the ‘garbage out’ category.

I’ve focused this article on quality research that has been peer-reviewed and published in legitimate journals, when possible. Anything referenced in a pre-print (non-peer-reviewed) publication will be clearly marked as such.

Genetics studies are just now beginning to come out on SARS-CoV-2 susceptibility, and I’m sure there will be many more to come! So consider this the beginning, not the final word, on genetics and SARS-CoV-2

Terminology:

Let’s define a few terms so that we are all on the same page:

SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is the name of the novel coronavirus identified by researchers in Wuhan, China in December 2019.
COVID-19 is the name for the disease/symptoms caused by the SARS-CoV2 virus.
Case: As everyone is probably aware, the definition of a COVID-19 ‘case’ is ever-changing and depends on the source. Numbers for cases differ according to each country’s definition, which also changes over time.[ref] This makes it difficult to make comparisons between countries and over time. Importantly, the varying definitions will impact the way that COVID-related genetic variants are identified.

Coronavirus Quick Facts:

Since the 1960s, researchers have identified more than 50 different coronaviruses, most of which only cause diseases in other animals. There are now 7 known human coronaviruses.
The viruses can be categorized as Alphacoronavirus or Betacoronavirus, depending on their structure. The common coronaviruses are one cause of the common cold.[ref]
The SARS-CoV-2 virus is a betacoronavirus, an enveloped virus with a single strand of RNA. It enters human cells through the ACE2 receptor protein on the cell surface. Inside the cell, it uses the host’s cellular machinery to replicate.[ref]
Previous serious viral outbreaks in the coronavirus-family include SARS-CoV and MERS-CoV.
Coronaviruses that cause the common cold make rounds every year in a seasonal respiratory virus pattern.

Spread of the virus:

The SARS-CoV-2 virus is airborne and spreads via exhaled, sneezed or coughed particles that contain the virus. Currently, almost all contact-tracing confirmed cases have spread in the home or in enclosed indoor environments that have poor air circulation. (In other words, outdoor spread or in well-ventilated spaces is not common.)[ref][pre-print ref]

Past coronavirus research clearly shows that cool, dry conditions facilitate the spread of the virus. This would include both winter-time weather as well as indoor, air-conditioned environments. Humid, warm environments decrease the spread (but only in areas where people don’t use the A/C when it gets hot). Studies in India show that SARS-CoV2 spreads quickly in cities with a strong power infrastructure and A/C usage, but in crowded cities with little air conditioning, the virus seems to not be spreading.[ref]

Asymptomatic cases:

Like most viruses, there is a huge variation in how sick people get with SARS-CoV-2. The majority of people are asymptomatic and don’t have symptoms. Of the people who do have symptoms (cases), the majority have cold-like symptoms. Unfortunately, a minority of people end up with severe symptoms that can lead to death, generally from either pneumonia or vascular complications.

The estimates on asymptomatic individuals range from initial studies showing 72% (Diamond Princess cruise ship) to initial serology studies showing 95% of people are asymptomatic or had symptoms mild enough not to be tested.[ref][pre-print ref] The CDC’s more recent estimates are that about half are asymptomatic or very mild, depending on which phone survey or serological study is used.[ref][ref]

Asymptomatic spread of the virus: While there is a lot of speculation about how often asymptomatic transmission of the virus occurs, research does show that asymptomatic carriers can spread the virus with close contact. One study looked at over 2000 close contacts of asymptomatic carriers and found the infection rate of close contacts with asymptomatic patients was 4%. [ref] It is likely that longer contact times allow more of a viral load to be transferred.

Pre-symptomatic spread is also well documented. Infected people can spread the virus a day or two before their symptoms begin.

The body’s first line of defense against respiratory viruses:

Our first line of defense against SARS-CoV-2 (and all airborne pathogens) is the cilia lining the nose and respiratory tract. The epithelial cells lining the upper respiratory tract can have as many as 200-300 cilia per cell, or little projections, on the surface. These cilia ‘beat’ or move back and forth in a coordinated fashion, moving pathogens and pollutants up and out of the airway. The mucous lining the surface collects the debris. The ciliary movement is thought to decrease in the elderly, and it is impaired by exposure to alcohol, cigarette smoke, and air pollution.[ref]

Nitric oxide is produced in the nose and helps to fight off pathogens. Researchers theorize that breathing through the nose rather than the mouth can decrease susceptibility.[ref]

ACE2 receptor:

What happens when the virus breaches the ‘first line of defense”? A virus can’t just wiggle its way into a cell. It must have a way of binding with a receptor on the cell surface and then bringing its RNA or DNA into the cell. Different viruses use different cell surface receptors to enter the cell.

Angiotensin-converting enzyme type 2 (ACE2 gene) is a receptor found on the surface of cells lining the lungs, nose, small intestines, blood vessels, and heart.[ref]
ACE2 is part of the renin-angiotensin-aldosterone system (RAAS), which controls blood pressure, fluid balance, and electrolytes in the body. ACE2 is considered to be an anti-inflammatory part of this system; it balances out the ACE1 enzyme.[ref]
Researchers theorize that low levels of ACE2 (due to older age, hypertension, diabetes, cardiovascular disease) are likely to increase lung inflammation and coagulation.[ref]
When the SARS-CoV-2 virus binds to ACE2 to enter the cell, the virus inactivates the ACE2 receptor. Researchers theorize that part of the hyperstimulation and cytokine storm produced by severe COVID-19 is due to diminished ACE2 production.[ref]

Some researchers theorize that inhibiting ACE1 will boost ACE2 and thus be beneficial in COVID-19 patients. ACE1 inhibitors often having coughing as a side effect, so angiotensin II receptor blockers (ARBs), such as the ‘-sartan’ blood pressure drugs, may be a better choice.[ref]

The research on whether more or less ACE2 is beneficial is still preliminary and not yet confirmed.

Antibodies, T-cell response, Complement System:

There are two main categories of the body’s immune system:

Innate Immune Response: jumps into action immediately and fights off a variety of pathogens.
Adaptive Immune Response: a more targeted approach that takes longer to initiate. The adaptive immune response can take days or weeks to develop a response to a specific pathogen. The advantage here is that the adaptive immune system creates a ‘memory’ of the pathogen and reacts immediately if exposed again.

With SARS-CoV-2 and other viruses, the innate immune response consists of interferon and other cytokines that can kill the cells containing the virus.

The adaptive immune response is unique to each virus and bacteria. In general, the adaptive immune response has two types of white blood cells:

B cells (or B lymphocytes): created in the bone marrow and able to produce antibodies specific to an invader
T cells (or T lymphocytes): maturing in the thymus and able to stimulate a variety of responses and long-term memory

Researchers are finding that while not everyone produces antibodies (from B cells) when they have COVID-19 (the majority do), some of the people who didn’t produce antibodies did produce T cells that fight and remember SARS-CoV-2.[pre-print]

Research from 2016 shows that T cell response was important in immunity for SARS-CoV and MERS-CoV, with antibodies fairly quickly disappearing for both of those viruses.[ref]

Not everyone produces antibodies to SARS-CoV2:
We hear a lot about antibody testing, but several recent studies show that some people who test positive for COVID-19 may not produce antibodies. The T-cell response may be more important for some people.

A preprint released on June 28th found that 80% of COVID-19 patients had IgG antibodies (leaving 20% without).[pre-print]
Antibodies may diminish very quickly in people who are asymptomatic. People with severe COVID-19 produced more antibodies than mild/moderate cases.[ref]
German research shows that the majority of COVID patients created a T-cell response, even if they didn’t produce antibodies (12% didn’t). Additionally, the study found that about 80% of the control group (patients without COVID) had T-cells from previous coronavirus exposure that cross-reacted to SARS-CoV2. This cross-reactivity may not provide complete immunity for everyone, but it may lessen symptoms.[pre-print] (Several previous studies had shown about 40-50% of the population has cross-reactive T-cells, likely from previous coronavirus exposures.)

Complement system

The innate immune system also includes the ‘complement system’. The complement particles circulate at low levels all the time in the bloodstream, and, when activated, cause inflammation and call in phagocytes to kill off pathogens.

The complement system is considered to be the oldest part of our immune system by evolutionary biologists. It is thought to be one of the first systems that evolved to identify bacteria as being different from the host.[ref]

There are several ways that the complement system can be activated. At low levels, it is always on patrol for pathogens – or anything that is not ‘self’ (known as the alternative pathway). Part of the complement system can enhance the immune response when antibodies are already present for the pathogen (known as the classical pathway). Finally, a part of the complement system is called mannose-binding lectin, which can bind to mannose on pathogens, marking the pathogen for destruction. Additionally, complement activation can increase clotting.

The complement system is composed of many different molecular components – some that recognize pathogens, some that cause activation of the immune response, and others that keep the complement system in check. Too much complement activation can result in the destruction of some of the body’s cells, such as happens in the eyes of elderly people with age-related macular degeneration. Another example of over activation of the complement system in young people is atypical hemolytic uremic syndrome, which causes damage to the endothelial cells lining the blood vessels.[ref]

An August 2020 paper in the journal Nature Medicine, explains that one big difference with severe COVID-19 patience may lay in activation, or rather, overactivation, of the complement system. The research was conducted using nasal swabs from patients at NYC hospitals and using genetic data from the UK Biobank.

The researchers found that people with age-related macular degeneration, a condition caused by a dysregulated complement system, were at a much higher risk of dying from COVID-19. Yes, the researchers took into account age and sex when determining whether age-related macular degeneration (ARMD) was a risk factor.

Additionally, the researchers found that people who were genetically more likely to have coagulation disorders (thrombosis, thrombocytopenia) were also more likely to have severe consequences from COVID-19. For example, the researchers identified an SNP in the factor 3 gene, which codes for part of the coagulation cascade, as increasing the risk of COVID-19 mortality by 2-fold. (listed in the genetics section below)

One more interesting study may add even more evidence to the idea that the complement system dysregulation is at the root of severe COVID-19. The study by Stanford researchers found that the immune response goes awry with severe COVID-19. What was interesting (in terms of activating the complement system) was the following quote:

“The scientists also found elevated levels of bacterial debris, such as bacterial DNA and cell-wall materials, in the blood of those COVID-19 patients with severe cases. The more debris, the sicker the patient — and the more pro-inflammatory substances circulating in his or her blood. The findings suggest that in cases of severe COVID-19, bacterial products ordinarily present only in places such as the gut, lungs, and throat may make their way into the bloodstream, kick-starting enhanced inflammation that is conveyed to all points via the circulatory system.”

Severe COVID-19 Genotype Report:

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Lifehacks for Boosting Immune Function:

A robust innate immune response (that initial response to a pathogen) is needed to clear the SARS-CoV-2 virus.

Below are a few of the research-backed ways to help stack the odds in your favor with the SARS-CoV-2 virus. These ‘lifehacks’ may help increase your immune response — but please know there are many variables, known and unknown, at play in whether you have symptoms with SARS-CoV-2.

No magic pills here… just support to keep your immune function in excellent condition.

Circadian rhythm and immune function:

Your immune system is better equipped to fight off pathogens during the day. This makes sense when you think about how society interacted before electricity — generally, people went to bed rather than going out on the town.

Animal studies show that the time of day matters a lot in viral replication. Mice infected with a virus right before the rest phase (e.g., bedtime) had a 10-fold greater viral replication than the mice infected at the start of their active phase. Additionally, mice with a core circadian rhythm gene knocked-out have a higher viral replication load, day or night.[ref]

The best thing for healthy people to prevent COVID-19 symptoms may end up being to avoid social activities at night and go to bed at a reasonable hour.

Jet lag also messes with your circadian rhythm and increases your susceptibility to viruses. Animal studies on jet lag clearly show increased susceptibility to viral bronchitis and increased replication of the virus.[ref] A similar circadian disruption occurs when people stay up later than usual, exposed to bright lights — or are switching over to working nights (such as healthcare workers). Read more about circadian rhythm and immune function.

Vitamin D:

Quite a few epidemiological studies have pointed out the link between vitamin D deficiency and susceptibility to more severe COVID-19.[ref][ref]

A vitamin D blood test is your best bet for knowing your levels, and spending some time out in the sun should help to boost your levels if needed.

What about vitamin D as a supplement? A recent cell line study tested a variety of known drugs and other compounds against SARS-CoV2. The study found several known prescription drugs (with major side effects), as well as calcitriol, a synthetic form of vitamin D3, was possibly effective against SARS-CoV2. Calcitriol stood out as being effective in several different types of cells, including nasal epithelial cells. Plus, it is inexpensive and safe.[pre-print ref]

Melatonin:

Released at night from the pineal gland, melatonin is vital to the immune system. Research studies on sepsis show that melatonin may be beneficial for reducing excessive cytokine production and restoring mitochondrial function. Many animal studies show that melatonin enhances immune function prior to stimulation, and it may also tamp down an excessive immune system response.[ref][ref][ref][ref]

You can boost your own body’s production of melatonin by eliminating exposure to light in the blue wavelengths at night. Light at night (at 480nm, blue light) triggers a receptor in the retina, causing melatonin production to be dampened. Either turn off electronics/lights or wear blue light-blocking glasses. Both will increase your endogenous melatonin production.

Will supplemental melatonin help? Quite a few researchers are suggesting that melatonin supplements are a good idea for combating COVID. Some suggest that supplemental melatonin be used as a prophylaxis by everyone to prevent COVID-19.[ref][ref] There is currently a clinical trial underway to determine the effectiveness of supplemental melatonin.[ref]

Dual histamine blockers (more severe cases):

Cetirizine plus famotidine: The hyperactive inflammatory response in severe COVID-19 patients was battled with cetirizine and famotidine in a hospital-based study. The results showed that the percentage of patients on ventilators and the mortality rate was lower for the patients (n=110) on the dual histamine blockade… but there was no official placebo group for comparison.[pre-print ref]

Why would this work? Blocking the histamine response should reduce the overactive inflammatory cascade. Histamine, a signaling molecule, is released by mast cells in response to pathogens (or allergens), but histamine also acts as a signal to trigger other mast cells to degranulate – thus worsening the overall inflammatory cascade.