Dante Labs: How to Download and Convert to 23andMe format

To use your Dante Labs whole-genome file on Genetic Lifehacks, you will need to convert the data into a format that looks like 23andMe or AncestryDNA raw data.

This article covers one way to do this conversion, but there are likely other options as well. I recommend doing this on a decent desktop or fast laptop computer (not a tablet or Chromebook…).

Download your whole genome file from Dante Labs:

1.) Log in to Dante Labs
Go to genome.dantelabs.com and log in.

2.) Under the Reports dropdown, choose Raw Data Library

3.) You’ll have a lot of options for different file formats. Click on BAM to download the BAM file. It is about a 50GB file, so it may take a while to download it.  (When I went to download my file just now, the file didn’t exist. I had to contact Dante Labs support about the error. Sigh. Hopefully, it goes smoothly for you.  ***Update from a member: Apparently you need to download the files within a few months of getting your genome sequenced. Otherwise they go into storage and you may have to pay a subscription fee to get access to the BAM file. )

 

Converting the BAM file using WGS Extract:

To convert the whole genome BAM file, I used WGS Extract, which is free, open-source software:

https://github.com/WGSExtract/WGSExtract.github.io

There is a detailed instruction manual available on the GitHub page (100+ pages).

Yep, this is one of those times that you will need to read the manual. The software is a bit rough around the edges when it comes to user interface and installation. The plus side is that the application is free, works well (I’m on a Mac), and did exactly what I needed.

Follow the installation instructions for your operating system.

Note: For the reference library, use hs37d5. It was option 13 in WGSE v3.

Once WGSE is installed and running:

1.) Select your BAM file.

2.) When you load in your Dante Labs BAM file, it will pop up an alert that it needs to be indexed.

Click on the Index button next to where it says Statistics and Attributes:

 

3.) Now, wait for 30 minutes or so to generate the BAM index file. (It didn’t take quite that long on my Mac.) You can do other things on your computer while you wait, but just don’t close the application or the terminal window.

For example, you can read through the manual again while you wait… :-)

4.) Key thing: once you’ve indexed the BAM file, you will need to click on the Stats button before you can do anything else. Yes, it clearly says this in the manual, but I missed it and was confused for a bit.

5.)Next, click on “Extract Data” and then on the “Microarray RAW” button.

To use the Genetic Lifehacks membership features, choose 23andMe v4, v5, and Ancestry v2. There looks like lots of other options to come back later and play with here.

 

6. Click the Generate button.

It will give you an expected wait – mine said 50 minutes, but it took about half that.

Your data files should now be in the folder that you set up for WGSE to use.

7. That’s it! You should be able to connect on the Member’s Start page to the new raw data file. If you are already using a different raw data file, you will need to hit the “Clear Data” button first before connecting to the new data file.

 

 

 

 

 




Author Information:   Debbie Moon
Debbie Moon is the founder of Genetic Lifehacks. She holds a Master of Science in Biological Sciences from Clemson University and an undergraduate degree in engineering from Colorado School of Mines. Debbie is a science communicator who is passionate about explaining evidence-based health information. Her goal with Genetic Lifehacks is to bridge the gap between the research hidden in scientific journals and everyone's ability to use that information. To contact Debbie, visit the contact page.