Should you eat organic? Detoxifying Neonicotinoids

Is buying organic worth the extra cost?

There isn’t a one-size-fits-all answer to that question.

Pesticides that are sprayed on conventionally grown foods affect people differently. Some people carry genetic variants that decrease their ability to detoxify specific pesticides, others may be more resilient.

This is Part One in a multipart series on pesticide detoxification.  I’m starting with what is considered to be a ‘safe’ pesticide – or at least a  better alternative to the more toxic pesticides – neonicotinoids. 

Background on Pesticides:

The term pesticide includes insecticides, herbicides, fungicides, antimicrobials, and bacteriocides.  These are generally sprayed on crops or used to coat the seeds to improve crop yields.

Startling study on infertility:

What started me down this path of investigating the relationship between genetic variants and the need to reduce pesticide exposure was a fertility study that was published a couple of years ago.

The study, published in JAMA, found that eating more fruits and vegetables that had high pesticide residue (conventionally grown) decreased the probability of getting pregnant.  Rather, it was the avoidance of pesticides – either through eating more organic fruits and vegetables or not eating fruits and vegetables – that increased fertility.

This study has bothered me for a while now.  It goes against the conventional thought that everyone should eat more veggies and get their ‘5 a day’.  Instead, it shows the negative impacts of pesticide residue may outweigh the positive benefits from the antioxidants and nutrients.

Neonicotinoids:

Neonicotinoids, also called neonics, are the most commonly used type of insecticide in the world.

Fields are sprayed with neonicotinoids, and they are also used to coat seeds before planting.  They were first introduced by Bayer in the early 1990s, and their usage skyrocketed in the 2000s.

Currently used neonicotinoids include:

  • imidacloprid
  • clothianidin
  • thiamethoxam
  • acetamiprid
  • thiacloprid

Neonics are generally considered safer than some of the other insecticide options. Seeds treated with the insecticide protect the seedling for the first few months of growth. This reduces the amount of other pesticides needed.  [ref]

How do they work?

Neonics work by damaging the insect’s nervous system – targeting the nicotine acetylcholine receptors. Nicotine is naturally produced by tobacco to fend off the bugs. Neonics are chemically similar to nicotine, but much more potent as an insecticide.[ref]

By targeting the insect’s nervous system, the insecticide can paralyze the insect and cause death.[ref]

The nicotinic acetylcholine receptors in insects are slightly different than what is found in vertebrate animals, including humans. This was the initial basis for assuming that the insecticides are safe for human consumption.[ref]

Environmental Impacts:

Recent studies have linked neonicotinoids to colony collapse disorder in bee colonies. The European Food Safety administration has called for a ban on them.[ref][ref]

Another environmental consequence of neonicotinoids is the impact on amphibians. It is thought that the neonics decrease their immune response to pathogens, leading to a widespread decline in some areas.  [ref]

Where are they used?

Neonics are commonly used to coat seeds.  A study on corn seed that was treated with the neonicotinoid, clothianidin, found that only 1.34%  was carried through to the rest of the plant tissue.[ref]

A 2014 study found that 72% of fruits, 45% of vegetables, and 90% of the honey sampled contained neonicotinoids. [ref]  So neonics are widely used and most people are exposed to them daily.

The USGS maps the data from water sampling showing where pesticides are used. You can look at maps for hundreds of pesticides. The two most commonly used neonicotinoids are imidacloprid and clothianidin.

Here is a map from the USGS water sampling showing clothianidin usage:

water.usgs.gov Estimated Agricultural Use for Clothianidin, 2016

Here is a map from the USGS water sampling showing imidacloprid usage.

water.usgs.gov Estimated Agricultural Use for Imidacloprid, 2016

A University of Iowa study found that neonicotinoids are widespread in surface water in agricultural areas.  The study found that neonicotinoids are only partially removed by water treatment systems, with the amount depending on the type of filtration.[ref] I wonder if the researchers who found that the University of Iowa’s filtration system only removed 1% of the clothianidin are still drinking from the water fountains there...

In general, neonics are widely used as seed treatment on sugar beets and cereal crops (corn, wheat, rice). They are also used as a spray for apple and pear trees and in lawn care. [ref]

Human studies:

So what happens in humans?  Surely there is no problem since the EPA says they are safe, right?

First, let’s look at how much people ingest/absorb:

Neonics are ingested with food or beverage consumption, absorbed through skin contact, inhaled if nearby to spraying, consumed by drinking contaminated water, or contacted through indoor dust. [ref]

Agricultural workers have high exposure levels of neonicotinoids through the skin. A recent study looked at urine samples from people who apply the pesticides. The results showed that 100% of the people had imidacloprid in their urine, both before and after they applied the pesticide to the fields.  The concentrations ranged from 0.21-8.91ng/mL before they went to work, to a range of 0.11 – 24.58ng/mL after they applied the pesticides.  The average increase was over 3-fold after applying pesticides. But you can see from the wide range that there is a huge difference from person to person.[ref]

Another study looked at the average amount of neonicotinoids found on foods (according to USDA data) and compiled it with the average food consumption data (NHANES data from 2012).

Researchers found that the estimated average daily intake was below the chronic reference dose allowed for imidacloprid. They conclude “However, due to their wide use, it is logical to expect the ubiquity of neonicotinoids in foods.”  [refI wonder what the effect would be of eating more vegetables than the standard American diet…

The study above used the data from the USDA pesticide database. Another website that uses the same database (in an easier to use format!) is called What’s on my Food.  It lists both the average amount found on different fruits and vegetables, the maximum daily limit, and the range of amounts found. You could use the What’s on My Food website and calculate your own daily consumption of different pesticides.

Another route of human exposure is through contact with a dog that has the flea medication Advantage on its fur. The study found that imidacloprid (which kills fleas) was detectable in the dog’s blood for 72 hours but it stayed on the fur for up to four weeks! The tests were done to see how much could be picked up by petting the dog for five minutes. The amounts dropped by about a third each week. [ref]

Human health problems:

Acute exposure to neonics causes human health problems such as skin and eye irritation, vomiting and nausea.[ref]

A study on neonicotinoids in tomatoes show that there is “relatively low bioaccessibility, indicating that the daily intake of the tomato can be regarded as being relatively safer.” It also notes that adding fiber to the diet reduces bioaccessibility by 18-67%. [ref]

A cell study on human breast cancer cells found that environmentally relevant doses of neonics cause changes to the CYP19 gene expression, similar to the changes seen in hormone-dependent breast cancer. [ref]

Another study using human lymphocyte cells found that imidacloprid (and two other pesticides) cause changes in a couple of specific chromosomes causing missegregation when dividing. [ref] This is a concern for cancer risk, but I’m not sure what the absolute impact would be.

Animals studies on neonicotinoids:

Since human studies are lackluster and scarce, let’s take a look at animal studies.

  • One neonicotinoid, thiacloprid, caused birth defects in baby chicks, especially at higher doses. [ref]
  • Repeated oral doses of imidacloprid (50 – 150 parts per billion) combines with low dose arsenic to cause testicular toxicity (in rats). [ref] (This could be a problem if a rice crop that is high in arsenic is sprayed with a neonic.)
  • A mouse study found that the neonicotinoid, imidacloprid, increased weight gain, fat mass, and insulin levels. [ref]
  • In wild birds, imidacloprid disrupts the thyroid levels. This was particularly important during reproduction for the wild birds. [ref]
  • The neonicotinoid, clothianidin, cause mice to behave anxiously at doses deemed safe (below the no observable adverse effect limit). [ref]

Metabolism of neonicotinoids (in humans):

In insects, neonicotinoids act on the nicotine acetylcholine receptors, causing death.  But what happens in human cells? We have a slightly different structure than insects have for the structure of the nicotine acetylcholine receptor.

In humans, activation of the nicotine acetylcholine receptors increases catecholamines (epinephrine, norepinephrine, etc). Overactivation of this system can cause rapid heartbeat, hypertension, vomiting etc.  A cell study shows that neonicotinoids activate tyrosine hydroxylase and PNMT. This, in turn, increased catecholamine production — but at a lesser level than nicotine does. [ref]  Basically, the neonicotinoids act similarly to nicotine, but at a much lower level. Animal studies back this up.[ref]

A study on agricultural workers found reduced acetylcholinesterase activity when exposed to a mixture of pesticides, including neonicotinoids. This then caused a reduction in SOD (superoxide dismutase) and catalase, which is a major way that the body fights against oxidative stress in cells. Decreased SOD and catalase can lead to tissue damage from oxidative stress. [ref]

To break down and get rid of neonicotinoids (imidacloprid in particular), your body uses the CYP3A4 enzyme.  This is an enzyme that also metabolizes about half of the prescription medications.  [ref][ref]

The neonic, clothianidin, was tested in human cells and found to down-regulate TNF-alpha and NF-κB, which are an important part of our immune defense system. This is thought to be the same mechanism by which the neonicotinoids are affecting honey bees (making them susceptible to viral pathogens).[ref] I don’t know how big an impact on humans the down-regulation of TNF-alpha would actually have. Sometimes down-regulation of TNF is good…


Genetic variants that impact neonicotinoid metabolism:

The genetic variants listed below impact the pathways for neonicotinoid metabolism and impact in the nervous system.

There are no specific studies (at least that I can find) that look at the impact of neonicotinoids on humans based on their genetic variants. In fact, there are few studies on humans and neonics in general — which is concerning considering we all consume them every day!

CHRNA4 gene variants:

The neonicotinoids clothianidin and imidacloprid are weak agonists of the human α4β2 nicotinic acetylcholine receptors. They bind to the receptors and inhibit the neuron’s response to acetylcholine – more so at higher amounts. This receptor is coded for by the CHRNA4 gene. [ref]

Check your genetic data for rs1044396 (23andMe v4, v5; AncestryDNA):

  • AA: protective against nicotine addiction, other addictions [ref][ref], possibly less affected by neonicotinoids
  • AG: protective against nicotine addiction, other addictions [ref][ref], possibly less affected by neonicotinoids
  • GG: normal function

CYP3A4 Gene Variants:

Studies show that imidazolidine is metabolized using the CYP3A4 enzyme. [ref] There are quite a few genetic variants that change the function of the CYP3A4 enzyme.  Note that if you have any of these variants, you should also read my article on CYP3A4 and how it impacts medications.

Check your genetic data for rs4987161 (23andMe v4, v5):

  • G/G: CYP3A4*17, decreased function of enzyme, [ref] [ref]
  • A/G: carrier of one CYP3A4*17 allele
  • A/A: normal

Check your genetic data for rs4986909 (23andMe v4; AncestryDNA):

  • A/A: CYP3A4*13, decreased function of the enzyme, [ref]
  • A/G: carrier of one CYP3A4*13 allele
  • G/G: normal

Check your genetic data for rs2740574 (23andMe v4,v5; AncestryDNA):

  • C/C: CYP3A4*1B, decreased function of the enzyme [ref]
  • C/T: carrier of one CYP3A4*1B allele
  • T/T: normal

Check your genetic data for rs4986910 (23andMe v4, v5):

  • G/G: CYP3A4*3, decreased function
  • A/G: carrier of one CYP3A4*3 allele
  • A/A: normal

Check your genetic data for rs4986907 (23andMe v4, v5):

  • T/T: CYP3A4*15A, decreased function
  • C/T: carrier of one CYP3A4*15A allele
  • C/C: normal

Lifehacks:

Buy Organic:

If you carry the CYP3A4 non-functioning variants, you may want to consider spending the extra money on organic products.

N-acetylcysteine:

A mouse study shows that n-acetylcysteine (NAC) partially reversed the effects of imidacloprid.  [ref]  NAC is a commonly used supplement and used to produce more glutathione (antioxidant).  It is also commonly used to help fight nicotine addiction, and studies show that it reduces cravings when quitting smoking. [ref][ref]

You can buy NAC in most health food stores or online through Amazon.  

Honey:

With the link between neonicotinoids and colony collapse, it should not come as a surprise that most honey has it in it.  One study found that 90% of honey samples had at least one neonicotinoid and 50% had two or more different neonicotinoids. [ref]  If you live in an area that doesn’t have a lot of agricultural fields around, your best bet is to get local honey at a farmer’s market where you can talk to the beekeeper.

Vitamin C:

One cell study showed that the antioxidant vitamin C helps to reduce the oxidative stress caused by imidacloprid.[ref]

Kids:

A couple of studies point out that children may be more susceptible to the negative effects of neonicotinoids. The nicotine acetylcholine receptors are important during brain development, and, similarly to nicotine, the neonics may be detrimental. [ref] This may be one more reason to feed your children (especially babies) organic foods when possible.

Questions left unanswered:

If almost all conventionally grown corn has neonicotinoids on it – and neonicotinoids act mildly like nicotine – does this explain my addiction to tortilla chips?


Enjoying what you are reading?  Consider supporting Genetic Lifehacks on Patreon.  This post was inspired by a survey of my patrons asking what topic they wanted to learn more about :-)

2 Comments on “Should you eat organic? Detoxifying Neonicotinoids

  1. Great info ! I have a variation on my SOD gene which makes it harder to breakdown toxins and to eliminate pesticides . People should check their genetics and never consume any unnatural foods !
    Txs again for the great info !

  2. I appreciate your thoroughness and efforts in the use of research based facts to help us navigate this tricky subject. Thank you for caring so much about our health and well being. Keep up the wonderful work.

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