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CYP2A6: Breaking down nicotine and other medications

Key takeaways:

  • CYP2A6 is an enzyme that breaks down nicotine as well as several important chemotherapy drugs.
  • Genetic variants in CYP2A6 can impact how well the enzyme functions, how certain chemo drugs work, and how hard it is to quit smoking.

This article is part of the Detoxification and Medication Genetic Lifehacks Hub.

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What is CYP2A6?

CYP2A6 is part of the CYP450 family of genes that are important in the phase I metabolism of medications and toxicants. CYP2A6 is involved in the breakdown (metabolism) of:

  • Nicotine
  • Tegafur (chemotherapy drug)
  • Letrozole (chemotherapy drug)
  • Efavirenz (antiretroviral)
  • Artemisinin (antimalarial)
  • Valproic acid (antiepileptic, bipolar, migraine med)
  • Pilocarpine (glaucoma and dry mouth)

Note that some drugs are metabolized through more than one CYP enzyme, so CYP2A6 may not be the only enzyme involved.

Endogenous substances metabolized by CYP2A6:

The CYP2A6 enzyme does more than just metabolize nicotine and certain medications. It also interacts with substances made naturally in the body.

CYP2A6 enzyme also breaks down:[ref]

  • retinoic acid (from vitamin A)
  • some steroid compounds

Estradiol, a form of estrogen, causes higher CYP2A6 activity. Thus, women usually have a somewhat higher overall activity of this enzyme.[ref]

Nicotine metabolism:

While other enzymes can also break down nicotine, the primary way that your body metabolizes it (breaks it down) is through hepatic CYP2A6 (70–80%). Also important to note is that the major metabolite of nicotine, called cotinine, is exclusively metabolized by the CYP2A6 enzyme.[ref] So, the CYP2A6 enzyme is responsible for two steps in the body’s process of getting rid of nicotine.

Quitting Smoking:
When it comes to smoking, the CYP2A6 genetic variants impact the speed at which nicotine is metabolized.

Researchers link genetic variants of CYP2A6 that cause decreased enzyme activity with a lower level of dependence in smokers, making it easier for them to quit. Basically, the slower CYP2A6 variants make the effects of nicotine last longer, thus reducing the number of cigarettes smoked.

On the other hand, people with increased CYP2A6 enzyme activity have an enhanced metabolism of nicotine and are likely to smoke more cigarettes per day.[ref]

Inhibiting CYP2A6 to reduce fibrosis:

While much of the research on CYP2A6 has focused on interactions with smoking, recent animal studies also show that it has a role in fibrosis (outside of cigarette smoke).

A 2025 animal study showed that inhibiting CYP2A with cinnamaldehyde, a component of cinnamon, significantly reduces liver fibrosis.[ref]


CYP2A6: Genotype Report

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Lifehacks:

Grapefruit juice:

Grapefruit juice contains a substance that inhibits CYP2A6 enzyme activity. So be careful – especially if you have a slow variant of the gene – with combining grapefruit juice with any drug that is metabolized by CYP2A6.[ref]

Cinnamon:

Higher doses of cinnamon, such as you might take to regulate blood sugar levels, have been shown to inhibit CYP2A6 enzyme activity.[ref] If you are a slower CYP2A6 metabolizer, you may want to be careful about too much cinnamon if you also take one of the medications listed below.

Which drugs are more effective for quitting smoking with slow CYP2A6?

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Related Articles and Topics:

CYP2C9: Genetic Variants and Drug Metabolism

Will statins give you muscle pain? SLCOB1 and ABCG2

CYP1A1: Detoxifying Cigarette Smoke and Estrogen


References:

Gao, Sicheng, et al. “Cinnamaldehyde Attenuates CCL4-Induced Liver Fibrosis by Inhibiting the CYP2A6/Notch3 Pathway.” Arab Journal of Gastroenterology, vol. 26, no. 3, Aug. 2025, pp. 262–71. DOI.org (Crossref), https://doi.org/10.1016/j.ajg.2025.04.001.

Chan, Jeannine, et al. “Inactivation of CYP2A6 by the Dietary Phenylpropanoid Trans-Cinnamic Aldehyde (Cinnamaldehyde) and Estimation of Interactions with Nicotine and Letrozole.” Drug Metabolism and Disposition, vol. 44, no. 4, Apr. 2016, pp. 534–43. PubMed Central, https://doi.org/10.1124/dmd.115.067942.
Chenoweth, Meghan J., Jennifer O’Loughlin, et al. “CYP2A6 Slow Nicotine Metabolism Is Associated with Increased Quitting by Adolescent Smokers.” Pharmacogenetics and Genomics, vol. 23, no. 4, Apr. 2013, pp. 232–35. PubMed, https://doi.org/10.1097/FPC.0b013e32835f834d.
Chenoweth, Meghan J., Andy Z. X. Zhu, et al. “Variation in P450 Oxidoreductase (POR) A503V and Flavin-Containing Monooxygenase (FMO)-3 E158K Is Associated with Minor Alterations in Nicotine Metabolism, but Does Not Alter Cigarette Consumption.” Pharmacogenetics and Genomics, vol. 24, no. 3, Mar. 2014, pp. 172–76. PubMed, https://doi.org/10.1097/FPC.0000000000000031.
“ClinPGx.” ClinPGx, https://www.clinpgx.org/gene/PA121?tabType=tabVip. Accessed 17 Feb. 2026.
Desta, Z., et al. “Plasma Letrozole Concentrations in Postmenopausal Women With Breast Cancer Are Associated With CYP2A6 Genetic Variants, Body Mass Index, and Age.” Clinical Pharmacology and Therapeutics, vol. 90, no. 5, Nov. 2011, pp. 693–700. PubMed Central, https://doi.org/10.1038/clpt.2011.174.
Di, Yuan Ming, et al. “Structure, Function, Regulation and Polymorphism of Human Cytochrome P450 2A6.” Current Drug Metabolism, vol. 10, no. 7, Sept. 2009, pp. 754–80. PubMed, https://doi.org/10.2174/138920009789895507.
Hanley, Michael J., et al. “The Effect of Grapefruit Juice on Drug Disposition.” Expert Opinion on Drug Metabolism & Toxicology, vol. 7, no. 3, Mar. 2011, pp. 267–86. PubMed Central, https://doi.org/10.1517/17425255.2011.553189.
López-Flores, Luis A., et al. “Distribution of Polymorphic Variants of CYP2A6 and Their Involvement in Nicotine Addiction.” EXCLI Journal, vol. 16, Mar. 2017, pp. 174–96. PubMed Central, https://doi.org/10.17179/excli2016-847.
Malaiyandi, V., et al. “Impact of CYP2A6 Genotype on Pretreatment Smoking Behaviour and Nicotine Levels from and Usage of Nicotine Replacement Therapy.” Molecular Psychiatry, vol. 11, no. 4, Apr. 2006, pp. 400–09. PubMed, https://doi.org/10.1038/sj.mp.4001794.
Tanner, Julie-Anne, and Rachel F. Tyndale. “Variation in CYP2A6 Activity and Personalized Medicine.” Journal of Personalized Medicine, vol. 7, no. 4, Dec. 2017, p. 18. PubMed Central, https://doi.org/10.3390/jpm7040018.

About the Author:
Debbie Moon is a biologist, engineer, author, and the founder of Genetic Lifehacks where she has helped thousands of members understand how to apply genetics to their diet, lifestyle, and health decisions. With more than 10 years of experience translating complex genetic research into practical health strategies, Debbie holds a BS in engineering from Colorado School of Mines and an MSc in biological sciences from Clemson University. She combines an engineering mindset with a biological systems approach to explain how genetic differences impact your optimal health.